I'd go for a 3 street bluff on this turn card against almost anyone, I think most ace high hands that don't have the As (obviously, since it's the turn) are going to fold a flop of this texture, and a lot of flush draws will c/r the flop. We can rep all the big hands here and he'll probably peel too often on the turn with KsJx type hands or 9x+spade, etc but never call it down for 3 streets. I think by the river we make him fold most non 2 pair hands which is basically his entire range besides A2/A9. That said, the Td river is basically the worst river in the deck to bluff, and it's still probably close to being a good bluff (although I'd be happier bluffing with 8 high or smth since we're still ahead of his QsJx,Js8x type hands with K5)

Gave it a fair bit of thought. I probably would 3-barrel, but considering T on the river, wouldn't it be somewhat likely that a thinking villain might deduce that we don't have an ace there (since we don't want to 3-barrel pair of aces on that scary river, but rather just check, right?), and be inclined to look us up with Kx, 9x, pocket pairs? What would be a good bet size for river if we decide to 3-barrel?

I'd usually lean towards a big river sizing since I always do with my value range as well and it tends to make sense since his range is weak here. If I bet 150 on the turn into 240 we get a 540 pot on the river and i'd bet 360-400. I agree with you about the Ax thing, although I'd still probably value bet AK here on the river he might not expect us to. That said, we can still have almost any Tx hand here so I don't think he should be looking us up with K high or always looking to call with 9x.